Characterization and Purification a Specific Xylanase Showing Arabinofuranosidase Activity from Streptomyces spp. 234P-16
Authors
ANJA MERYANDINI, TRIO HENDARWIN, FAHRRUROZI FAHRRUROZI, ALINA AKHDIYA, DEDEN SAPRUDIN, YULIN LESTARIFiles
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Journal
Published in Vol. 10 No. 3 (2009) of Biodiversitas Journal of Biological Diversity.
Biodiversitas encourages submission of manuscripts dealing with all biodiversity aspects of plants, animals and microbes at the level of the gene, species, and ecosystem as well as ethnobiology. Scopus (Q2)
About the JournalAbstract
Streptomyces spp 234P-16 producing xylanase was isolated from soil sample from Padang, West Sumatra, Indonesia. Crude enzyme (produced by centrifuging the culture at 14000 rpm for about 5 minutes) and purified xylanase have an optimum condition at pH 5 and 90oC. Crude xylanase have half life time of 4 hours, whereas purified xylanase have half life time of 2.5 hours at 90oC. The molecular mass of purified xylanase was determined to be 42.4 kDa. The Arabinofuranosidase have a Km and Vmax value of 1.98 mg/mL and 523 μmol/minute/mg, respectively
Key words: xylanase, xylooligosaccharide, arabinofuranosidase, Streptomyces.
Key words: xylanase, xylooligosaccharide, arabinofuranosidase, Streptomyces.
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