Identification of polymorphism on ND-5 mitochondrial DNA fragment of Benggala and Madura cattle with PCR-RFLP technique
Authors
NEO ENDRA LELANA, SUTARNO SUTARNO, NITA ETIKAWATIFiles
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Journal
Published in Vol. 4 No. 1 (2003) of Biodiversitas Journal of Biological Diversity.
Biodiversitas encourages submission of manuscripts dealing with all biodiversity aspects of plants, animals and microbes at the level of the gene, species, and ecosystem as well as ethnobiology. Scopus (Q2)
About the JournalAbstract
The objectives of the research were to detect genetic variations on ND-5 region of mtDNA of Benggala and Madura cattles, and to compare the genetic diversity within or between Madura and Benggala cattle. Genetic variations and its effects on phenotype characters have been studied largely in dairy cattles, but not for beef cattles, especially for Indonesian local cattles. PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) was used to detect polymorphism on ND-5 region of mitochondrial DNA. Polymorphisms were found on ND-5 mitochondrial DNA fragment using HindIII restriction enzyme. This variation were likely due to lost of HindIII restriction site on ND-5 mithocondrial DNA fragment.
© 2003 Jurusan Biologi FMIPA UNS Surakarta
Key words: ND-5 mitochondrial DNA, genetic variation, PCR-RFLP.
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